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If you exit the Unity3D Editor when a scene was saved in isolation mode the objects will remain isolated when you open the scene in your next session. Alternatively, when you run an Isolation in one scene, and then try to de-isolate those objects in a different scene, the data to de-isolate objects in your first scene will be lost. In this case, use the ‘De-isolate all objects in Scene' function to get back all objects in your original scene.